- 中文名称
MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit
- 英文名字
- MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- M2690-17
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Monocyte chemotactic and activating factor (MCAF), also known as monocyte chemotactic protein 1 (MCP-1), lymphocyte–derived chemotactic factor (LDCF), and glioma-derived chemotactic factor (GDF), is a recently-identified chemotactic cytokine for monocytes. cDNA cloning and structural analysis has revealed that this 76-amino acid polypeptide with a predicted molecular mass of 8,700 daltons belongs to a family of structurally-related low molecular weight proteins characterized by four conserved cysteine residues designated C-C family or intercrine beta family (1,2,3).This MCAF ELISA is a 2.5 hour solid-phase immunoassay readily applicable to measure MCAF levels in serum, plasma, cell culture supernatant and other biological fluids in the range of 0-1600pg/ml. It has shown no crossreactivity with various other C-C and C-X-C chemokines, IL-8 superfamily proteins. This MCAF ELISA is expected to be effectively used for futher investigation into the relationship between MCAF and various diseases.This MCAF enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for MCAF. Standards or samples are then added to the appropriate microtiter plate wells and incubated. MCAF if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound MCAF and other components of the sample. In order to quantify the amount of MCAF present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for MCAF is added to each well to sandwich the MCAF immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MCAF and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.In order to measure the concentration of MCAF in the samples, this kit contains two calibration diluents (Calibrator Diluent 1 for serum/plasma testing and Calibrator Diluent 2 for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus MCAF concentration (pg/ml). The concentration of MCAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: < 4pg/mlRange: 0-1600pg/mlKit Components:1. MCAF Microtiter Plate, 1x96 wells2. MCAF Conjugate, 1x15ml3. MCAF Standard, 2x1 vial 4. Calibrator Diluent 1, 1x22ml5. Calibrator Diluent 2, 1x22ml6. Wash Buffer (20X), 1x60ml7. Substrate A, 1x11ml8. Substrate , 1x11ml9. Stop Solution, 2N Sulphuric Acid (H2SO4). Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到MCAF, Human (Monocyte Chemotactic Activating Factor) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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