WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 3.5 µg/mL is recommended for WB. The predicted length of human actinin (isoform #1) is 892 residues and a MW of 103 kDa. A concentration of 3.5-7.0 µg/mL is recommended to detect alpha actinin in formalin fixed and paraffin embedded tissues. Antigen retrieval is required. Also the recommended titre for formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.