WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.50 µg/mL is recommended for WB. Bovine Calcineurin alpha has a predicted length of 521 aa and MW of 59 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect Calcineurin alpha in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.