WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human caldesmon has a predicted length of 793 residues and a MW of 93 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect caldesmon in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.