WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25-0.50 µg/mL is recommended for WB. Human calponin has a predicted length of 297 aa and a MW of 34 kDa. A concentration of 0.5-1.0 µg/mL is recommended to detect calponin in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.