WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Bcl-2 has a predicted length of 239 aa and MW of 26 kDa. A concentration of 0.4-1.0 µg/mL is recommended to detect Bcl-2 in formalin fixed and paraffin embedded tissues as well as formalin/acetone frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 from human Bcl-2 conjugated to thyroglobin.