IH,Frozen; IC; Immunohistochemistry (IHC) and immunocytochemistry (ICC): 1-2 µg/mL. This antibody has been shown to work on 4% PFA fixed mouse brain sections.
Western blotting (WB): 0.5-1.0 µg/mL. This antibody detects bands between 50-65 kDa, which only appear in stimulated cells.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Full length, E.coli-derived recombinant human c-FOS protein.