WB; IHC, Frozen; ELISA Western Blotting (WB), Immunohistochemistry (IHC) and ELISA. Suggested dilutions for WB at 1:500 - 1:1000, ELISA at 1:5,000-10,000 and IHC at 1:50-1:100. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.