- 中文名称
Sheep Hemagglutination Kit - KPA-3913
- 英文名字
- Sheep Hemagglutination Kit - KPA-3913
- 供应商
- Rockland
- 产品货号
- KPA-3913
- 产品报价
- ￥询价/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- Hemagglutination method is used for titer evaluation of viruses (e.g. Influenza, COVID-19 etc.), bacteria (e.g. Staphylococci, Shigella etc.), proteins (e.g. certain glycoproteins such as Lectins) and other agents which have the ability to attach to red blood cells (RBC)’s surface molecules.
For example, the influenza viruses agglutinate RBCs through their interaction with sialic acid receptors on the host cells. At a molecular level, viral hemagglutinins bind to sialic acid linked to galactose residues through alpha 2.3 or 2.6 linkage, which varies from species to species.
Once a hemagglutinin agglutinates the RBCs, each of the agglutinating molecule attaches to RBCs to form a lattice-structure or a meshwork. This prevents RBCs from settling down from suspension in a round bottom 96 well plate. Visually, a hemagglutinated sample will generate a uniform red colored suspension while a sample lacking hemagglutination will lead to settling down of RBCs to the bottom of wells to form button or dot shape. In viral hemagglutination assay, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.05% -0.1%) for 30 minutes to an hour at room temperature or 4° C. Thereafter, lattice formation is observed and the titer of a hemagglutination assay can be determined by the last viable "lattice" structure found.
If diluted further, the amount of Virus particles will be less than the RBCs and thus not be able to agglutinate them.
A Hemagglutination-inhibition (HAI) assay will involve titration of the viral hemagglutination with an anti-viral antibody (often from serum of human or animal infected with that virus) for inhibition of hemagglutination (i.e. neutralization of virus). HAI is one of the most commonly used methods to quantify immunity from influenza and other respiratory viral disease vaccines and is considered the gold standard as a correlate of vaccination mediated protection.
- 产品描述
- 10X Phosphate Buffered Saline (PBS) is a concentrated stock solution and should be diluted appropriately with distilled, deionized water or equivalent to its final working concentration which is 1X for this kit.
SHEEP RED BLOOD CELLS (RBC) 10% Washed Pooled Cells - R405-0005 should be diluted in 1X PBS to 0.05% concentration.
Anti-SHEEP Red Blood Cell (RBC) (RABBIT) Antibody - 213-4139-0002 binds and thus hemagglutinate Sheep RBCs when used at a dilution range of 1:25-1:50. An antibody mediated hemagglutination control is useful as a qualitative index for the integrity of the RBCs in the assay. Further serial dilutions up to 1:400 can be included in the assay if an independent or antibody-based control is required in the assay.
- 产品特点
- 该Sheep Hemagglutination Kit - KPA-3913的详细信息查看Rockland提供的产品说明书。
- 保存建议
- Store sheep washed pooled red blood cells at 4° C prior to opening. Be advised that blood is a perishable product and exact shelf may depend on application.
- 其他
- Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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