- 中文名称
Mouse Endostatin AccuSignal ELISA Kit - KOA0834
- 英文名字
- Mouse Endostatin AccuSignal ELISA Kit - KOA0834
- 供应商
- Rockland
- 产品货号
- KOA0834
- 产品报价
- ¥询价/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 90%产品中国有现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.
- 产品描述
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Endostatin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Endostatin standard solution and each sample be measured in duplicate.
- 产品特点
- 该Mouse Endostatin AccuSignal ELISA Kit - KOA0834的详细信息查看Rockland提供的产品说明书。
- 保存建议
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- 其他
- Rockland成立于1962年,是一家老牌抗体原产企业,其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体,包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素(Ubiquitin)等识别抗体,应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外,Rockland也生产特别齐全的二抗,包括纯化、生物素、HRP/ALP、荧光标记二抗,应用十分广泛。
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- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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