Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for AKT3 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT3 protein. AKT3 Western Bloting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.
应用类型
Western Blot,
免疫原
该【干冰】AKT3 Western Blot Kit - KCAE75的详细信息查看Rockland提供的产品说明书。
