- 中文名称
Interleukin 16, Human (LCF, prIL-16, FLJ16806, FLJ42735, FLJ44234, HST19289 ) BioAssay ELISA Kit
- 英文名字
- Interleukin 16, Human (LCF, prIL-16, FLJ16806, FLJ42735, FLJ44234, HST19289 ) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- I8438-04
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Introduction:Interleukin-16 (IL-16) discovered in 19821, known formerly as lymphocyte chemoattractant factor (LCF) is a lymphocyte chemoattractant factor of T cell origin with selective activity for CD4+ T cells. LCF was designated interleukin-16 (IL-16) in 1995. IL-16 is a product of CD8+ cells, CD4+ cells2, eosinophils3, mast cells and epithelial cells derived from asthmatics4. Expression of IL-16 in asthmatic epithelium correlates with the number of infiltrating CD4+ T cells5. LCF was originally identified and purified from the supernatants of ConA-stimulated peripheral blood mononuclear cells (PBMC). In addition to its chemotactic activity, it is a competence growth factor selective for CD4+ T cells dependent on an interaction with CD4 for induction of functional activity. Its cDNA codes for a novel 14-kD protein into a homotetrameric form is required for induction of biologic activity. IL-16 appears in culture supernatants as a relative molecular mass (Mr) ~56,000 biologically active, non-covalently linked tetramer, but migrates in monomeric form in SDS PAGE. Eluted monomeric peptides are inactive but reaggregate to Mr 56,000 regaining biological activity6. The protein expressed from the IL-16 cDNA demonstrates all the functions and chemical features of the native protein, including an identical pI, and autoaggregation into functional tetramers.IL-16 is preformed and stored in biologically active form in CD8+. T cells from which it is secreted following stimulation with histamine via H2 type receptors. The secretory process occurs within 4 hrs and does not require transcription, translation, or new protein synthesis.IL-16’s ability to inhibit the MLR and other antigen induced activation suggests that it may be useful in inhibiting allograft rejection. Because IL-16 selectively induces IL-2 responsiveness in CD4+ T cells it may be useful (along with IL-2) for selective CD4+ T cell immune reconstitution in individuals with lymphopenia, for example following chemotherapy or HIV-1 infection. In the latter circumstance, its ability to inhibit HIV transcription should provide a protective role if used as a therapy in HIV-1 infected individuals7. Inhibitors of IL-16 chemotactic function may be useful in diseases in which it appears to play a prominent role in the inflammatory process. The presence of IL-16 early after antigen challenge in asthma8, the marked upregulation of IL-16 synthesis by epithelium of asthmatics along with the correlation of IL-16 protein with the number of infiltrating CD4+ T cells in the airways of human asthmatics suggest that therapeutics aims at blocking IL-16 synthesis or function may be valuable in this disease.This IL-16 ELISA is a 4.5 hour solid phase immunoassay readily applicable to measure IL-16 in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 3200pg/ml. It showed no cross-reactivity with other cytokines tested such as EGF, EPO, GM-CSF, IL-1b, IL-7, IL-8, IFN-g, MCAF, MCP-3, M-CSF, SAA, TGF-b, and TNF-a.Principle of the Assay:This IL-16 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-16. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-16 if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound IL-16 and other components of sample. In order to quantitate the amount of IL-16 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for IL-16 is added to each well to sandwich the IL-16 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate solution are allowed to react over a short incubation period. Only those wells that contain IL-16 and enzyme-substrate reaction will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change measured spectrophotometrically at a wavelength of 450nm ± 2nm. In order to measure the concentration of IL-16 in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-16 (pg/ml). The concentration of IL-16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.Kit Components:1. IL-16 Microtiter Plate, 1x96 wells2. IL-16 Conjugate, 1x25ml3. IL-16 Standard, 2x1 vials4. Calibrator Diluent I, 1x30ml 5. Calibrator Diluent II, 1x50ml 6. Wash Buffer (20X), 1x 60ml 7. Substrate A, 1x16ml 8. Substrate B, 1x16ml9. Stop Solution, 1x14ml, 2N Sulphuric Acid (H2SO4). Sensitivity: <20ng/mlRange: 20-3200ng/mlStorage and Stabilty:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Interleukin 16, Human (LCF, prIL-16, FLJ16806, FLJ42735, FLJ44234, HST19289 ) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin 16, Human (LCF, prIL-16, FLJ16806, FLJ42735, FLJ44234, HST19289 ) BioAssay ELISA Kit产品后将其置于-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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