- 中文名称
Interleukin 4, Human (IL-4) BioAssay ELISA Kit
- 英文名字
- Interleukin 4, Human (IL-4) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- I8426-01
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 购买方式
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Principle:This IL-4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-4 and incubated. IL-4 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be sandwiched by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-4 and other components of the sample. In order to quantitate the amount of IL-4 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-4, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.Sensitivity: Calibrator Diluent I: 6.6pg/mlCalibrator Diluent II: 4.3pg/mlRange: 31.25pg/ml to 1000pg/mlKit Components: I8426-01M: Microtiter Plate 1x96wellsI8426-01N: Mab (Biotin) 1x7mlI8426-01P: Avidin (HRP) 1x14ml*I8426-01Q: Standard 2x1vialI8426-01R: Calibrator Diluent I 1x22ml For serum/plasma testingI8426-01S: Calibrator Diluent II 1x22ml For culture supernatantI8426-01T: Wash Buffer, 20X 1x60mlI8426-01U: Substrate A 1x10mlI8426-01V: Substrate B 1x10mlI8426-01W: Stop Solution 1x14mlSpecificity:This sandwich ELISA recognizes both natural and recombinant human IL-4, exhibiting no detectable crossreactivity with human; SAA, EGF, IL-8, IL-16, MCAF, MCP-3, TNF-g, M-CSF, GM-CSF, TGF-b1, RANTES, FGF, MIP-1a, CRP, EPO, IFN-g.Storage and Stability:Store *I8426-01Q: Standard powder at 4°C, liquid stock at -20°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Interleukin 4, Human (IL-4) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Interleukin 4, Human (IL-4) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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