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细胞分析试剂盒
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中文名称

Immunoglobulins ELISA Kit, Hamster, BioAssay

英文名字
Immunoglobulins ELISA Kit, Hamster, BioAssay
供应商
USBiological
产品货号
I7502-10
产品报价
¥1/1Kit
产品说明书
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产品新闻
背景资料
USBiological品牌是全球有名的抗原抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理,是行业中少有的致力于服务客户,帮助客户,且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应,专业的产品和解决方案支持,稳健快捷的交货周期,优质放心的售后服务。我们致力于为您提供有价值的产品和服务,在意您的成功!
产品描述
Immunoassays using enzyme labeled antibodies are highly specific for the analysis of a particular protein. Use of an enzyme labeled antibody together with a highly sensitive TMB substrate provides an excellent method for the detection and characterization of sample proteins bound to solid surface by ELISA techniques. Following attachment of protein to the solid surface (ELISA plate), a primary antibody is used to selectively bind the protein of interest. Alternatively, a known protein is bound to the surface for the screening of specific monoclonal/polyclonal antibodies in serum or other samples. An enzyme labeled second antibody directed against the primary antibody is then applied. The TMB substrate reacts with bound HRP to produce blue color. Upon addition of stop solution, the color is converted to yellow color (measure at 450nm). The yellow color is more intense and thus more sensitive than the blue color. Intensity of color, up to a certain range, is directly proportional to the amount of bound primary antibody.This ELISA kit is a qualitative, direct antigen-based assay designed to detect the presence of hamster antibodies in serum and to determine antibody titer using antigen-coated plates. It is not suitable for the quantitation of hamster IgG. The HRP-conjugated anti-hamster IgG provided in this kit is made against highly pure, whole antigen molecule specific to both H and L chains and will detect all IgG's (IgG + IgA + IgM). The reagents contained in this kit are sufficient for 1000 to 2000 samples or 10-20 plates.Kit Components:I7502-10A: IgG (HRP) anti-hamster 1x150ul. I7502-10B: Coating Buffer, 10X, 1x25 ml. I7502-10C: Blocking Buffer, 10X, 1x25ml. I7502-10D: Sample/Conjugate Diluent, 10X, 1x25ml. I7502-10E: Wash Buffer, 100X, 1x25ml.I7502-10F: TMB Substrate, 1x100ml.I7502-10G: Stop Solution, 10X, 1x10ml. Storage and Stability:Store all kit components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
产品特点
针对Immunoglobulins ELISA Kit, Hamster, BioAssay该产品的特点优势,欢迎查阅官网提供的产品说明书。
保存建议
建议收到Immunoglobulins ELISA Kit, Hamster, BioAssay产品后将其置于4°C保存。
其他
Usbiological公司是美国著名的抗体和生化试剂供应商,生产世界上种类最多的抗体,用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品,为科研用户提供了诸多超值选择。
注意
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