DnaK， originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.The protein coding region of the substrate binding domain of DnaK （amino acids 385-638） was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid （Met） is attached at N- terminus.
序列
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来源宿主
大肠杆菌中重组表达
溶解建议
Sterile filtered colorless solution. The DnaK protein contains 25mM Tris-HCl， pH7.5， 100mM NaCl， 5mM DTT and 10%Glycerol.
保存建议
Store at 4℃ if entire vial will be used within 2-4 weeks.
Store， frozen at -20℃ for longer periods of time.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Avoid multiple freeze-thaw cycles.
