DnaK， originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak（residues 508-638） of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft. DnaK（amino acid 508-638） was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid （Met） is attached at N- terminus.
序列
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大肠杆菌中重组表达
溶解建议
Sterile filtered colorless solution. The protein contains 25mM Tris-HCl， pH7.5， 100mM NaCl， 5mM DTT and 10%Glycerol.
保存建议
Store at 4℃ if entire vial will be used within 2-4 weeks.
Store， frozen at -20℃ for longer periods of time.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Avoid multiple freeze-thaw cycles.
