该IGFBP-1抗体是通过用衍生自与白喉类毒素连接的人IGFBP-1的中心结构域的12个氨基酸的肽免疫兔而制备的。 该肽序列位于甲状腺球蛋白1型结构域的紧邻上游,是IGFBP-1所特有的。 该抗体识别人IGFBP-1,但不识别IGFBP-2、3、4、5或6或相关蛋白。 尽管该序列对人IGFBP-1是特异性的,但该抗体确实识别大鼠和小鼠IGFBP-1,并且也可在许多其它物种中起作用,尽管这应通过实验确定。 通过蛋白A层析纯化免疫球蛋白级分。 该抗体适用于免疫测定、免疫印迹和免疫组织化学。 This IGFBP-1 antibody was prepared by immunising rabbits with a 12 amino acid peptide derived from the central domain of human IGFBP-1 linked to diphtheria toxoid. The peptide sequence is located immediately upstream of the thyroglobulin type-1 domain and is unique to IGFBP-1. The antibody recognises human IGFBP-1 but not IGFBP-2, 3, 4, 5 or 6 or related proteins. Although the sequence is specific to human IGFBP-1, the antibody does recognise rat and mouse IGFBP-1 and may also work in a number of other species, although this should be experimentally determined. The immunoglobulin fraction was purified by Protein A chromatography. The antibody is suitable for use in immunoassay, immunoblotting and immunohistochemistry.
GroPep Bioreagents公司总部位于澳大利亚,成立于1990年,致力于向广大科研院所、生物技术及制药公司开发、生产和销售细胞培养级的活性生长因子(主要集中在胰岛素样生长因子(IGF)领域)、抗体及相关蛋白试剂,现有产品超过100多个,同时在世界范围内均设有分销商。公司在分子生物领域、发酵学及重组蛋白和单抗制备方面具有丰富的经验,在制造工艺方面,拥有大量专利技术,GroPep目前已通过AS/NZS ISO 9001:2016质量管理体系认证。