鲑鱼/鳟鱼IGF-II抗体通过用对应于成熟大西洋鲑鱼N-末端氨基酸残基1 - 9的合成肽表位免疫兔来制备( 鲑)胰岛素样生长因子-II。收获抗血清,并通过疏水电荷诱导层析部分纯化IgG级分。该抗体对来自大西洋鲑鱼和虹鳟鱼的IGF-II具有高度特异性( 梅氏虹鳟)在酶联免疫测定中。与鲑鱼胰岛素的交叉反应性约为5%,<0.01%对鲑鱼IGF-I和鲑鱼GH的影响。 nSalmon/Trout IGF-II antibody was prepared by immunising rabbits with a synthetic peptide epitope corresponding to amino acid residues 1-9 of the N-terminus of mature Atlantic salmon (Salmo salar) IGF-II. The antiserum was harvested and the IgG fraction partially purified by hydrophobic charge induction chromatography. The antibody is highly specific for IGF-II from Atlantic salmon and Rainbow trout (Oncorhynchus mykiss) in an enzyme-linked immunoassay. It has a cross-reactivity of ~ 5% to salmon insulin and < 0.01% to salmon IGF-I and salmon GH.
GroPep Bioreagents公司总部位于澳大利亚,成立于1990年,致力于向广大科研院所、生物技术及制药公司开发、生产和销售细胞培养级的活性生长因子(主要集中在胰岛素样生长因子(IGF)领域)、抗体及相关蛋白试剂,现有产品超过100多个,同时在世界范围内均设有分销商。公司在分子生物领域、发酵学及重组蛋白和单抗制备方面具有丰富的经验,在制造工艺方面,拥有大量专利技术,GroPep目前已通过AS/NZS ISO 9001:2016质量管理体系认证。