- 中文名称
Glutathione (GSH) Assay Kit, BioAssay
- 英文名字
- Glutathione (GSH) Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- G8123-47
- 产品报价
- ￥1/250Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase.Simple, direct and automation-ready procedures for measuring reduced glutathione are becoming popular in Research and Drug Discovery. Glutathione Assay Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5'-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and detection (Reagent A) into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412nm, is directly proportional to glutathione concentration in the sample. The optimized formulation has a long shelf life and is completely free of interference due to sample turbidity.Key Features:Sensitive and accurate. Linear detection range 0.4-100uM in 96-well plate.Simple and convenient. The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density.Low interference. Amino acids and common buffers do not interfere.Applications:Direct Assays: reduced glutathione in whole blood, plasma, serum, urine, tissue and cell extracts.Drug Discovery/Pharmacology: effects of drugs on glutathione metabolism.Kit Components: (250 tests in 96-well plates)G8123-47A: Reagent A, 1x30ml G8123-47B: Reagent B, 1x30ml G8123-47C: Calibrator, 100uM, 1x10ml (equivalent to 100uM glutathione)Storage and Stability:Store components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Protocol:Important: equilibrate Reagents to room temperature. Shake Reagent A before use.Samples: whole blood samples should be diluted 20-fold with water prior to the assay (n=20). Cell lysate can be prepared as follows: collect 2 x 106 cells by centrifugation at 1,000g for 10 min at 4°C. Wash cells in cold PBS. Lyse cells by homogenization or sonication in 1-2ml of cold buffer containing 50mM MES or phosphate (pH 6-7) and 1mM EDTA. Centrifuge at 10,000g for 15 min at 4°C. Use supernatant for assay.Note: b-mercaptoethanol, dithiothreitol and cysteine are known to interfere in this assay. Avoid using these compounds during sample preparation. Amino acids do not interfere.Procedure using 96-well plate:1. Blank and Calibrator. Transfer 100ul water and 100ul Calibrator into wells of a clear-bottom 96-well plate. Pipette 200ul water into the Blank and Calibrator wells.2. Samples. Whole blood samples should be diluted 20-fold with water prior to the assay (n=20). Deproteination is required for blood, serum, plasma and other proteinaceous samples. Reagent A contains components for both color reaction and deproteination.Mix 120ul sample with 120ul Reagent A in 1.5-mL centrifuge tubes. Vortex to mix well. If turbidity occurs, pellet 5 min at 14,000 rpm in a table centrifuge. If the mixture remains clear, no centrifugation is necessary.3. Transfer 200ul sample/Reagent A mixture into wells of the 96- well plate. Add 100ul Reagent B. Tap plate lightly to mix.4. Incubate 25 min at room temperature. Read OD412nm.Procedure using Cuvet:Mix 400ul sample with 400ul Reagent A, centrifuge sample tubes if precipitation occurs. Transfer 600ul supernatant and mix with 400ul Reagent B. Incubate 25 min at room temperature. Measure OD412nm against water. Transfer 400ul Calibrator and 800ul Water into a clean cuvet and measure OD412nm against water.Calculation:Subtract blank OD (water) from the Calibrator and Sample OD values. The glutathione concentration of Sample is calculated as=ODSAMPLE–ODBLANKODCALIBRATOR–ODBLANKX 100 x n (uM)ODSAMPLE, ODSTD and ODBLANK are optical density values of the sample, Calibrator and sample “Blank” (water or buffer in which the sample was dissolved). n is the dilution factor (20 for blood samples).Conversions: 1 mg/dL glutathione equals 32.5uM, 0.001% or 10 ppm.Materials Required, But Not Provided:Pipeting devices, centrifuge tube and table centrifuge.Procedure using 96-well plate:Clear bottom 96-well plates (e.g. Corning Costar) and plate reader.Procedure using cuvette:Spectrophotometer and cuvets for measuring OD at 412 nm.Example:20ul fresh mouse blood was mixed quickly with 380ul water. Assays in 96-well plate gave blood glutathione concentration of 1124 ± 8uM (n=2)
- 产品特点
- 针对Glutathione (GSH) Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Glutathione (GSH) Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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