- 中文名称
FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)
- 英文名字
- FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)
- 供应商
- USBiological
- 产品货号
- F9103-52
- 产品报价
- ￥1/100Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- FTO, Fat mass-and obesity-associated gene, was discovered as a responsible gene causing the mouse 'fused toes' mutation. The predicted 502aa Fto protein has a calculated molecular mass of 58kD and contains an N-terminal bipartite nuclear localization signal. RT-PCR detected Fto expression throughout mouse embryonic development and in all adult mouse tissues examined except heart and skin. Bioinformatics analysis indicates that FTO shares sequence motifs with iron- and 2-oxoglutarate (2OG)-dependent oxygenases. Recombinant murine Fto catalyzes the iron- and 2OG-dependent demethylation of 3-methylthymine in single-stranded DNA, with concomitant production of succinate, formaldehyde, and carbon dioxide. In wildtype mice, Fto mRNA is most abundant in the brain, particularly in hypothalamic nuclei, and are regulated by feeding and fasting. This assay is a sandwich ELISA for quantitative determination of mouse FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of FTO in the samples. This ELISA is specific for the measurement of natural and recombinant mouse FTO. It does cross-react with mouse adiponectin, mouse RBP4, mouse Nampt, mouse vaspin, mouse progranulin, mouse resistin, mouse clusterin, mouse GPX3, mouse CD137, rat adiponectin, rat Nampt, human adiponectin, human Nampt, human progranulin. The assay range is 0.156-10ng FTO/ml and a detection limit of 20pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).Sample Type:Cell lysates.Intended Use:For the quantitative determination of mouse FTO in cells.Sensitivity:20pg/ml Range:0.156-10ng FTO/mlSpecificity:MammalianTest Principle:This assay is a sandwich ELISA for quantitative determination of mouse FTO in cells. A monoclonal antibody specific for FTO has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, FTO is recognized by the addition of a purified polyclonal antibody specific for FTO (Detection Antibody). After removal of excess polyclonal antibody, HRP conjugated anti-rabbit IgG (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3',5,5'-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450nm after acidification and is directly proportional to the concentration of FTO in the samples.Kit Components:Pre-coated Microtiter Plate: 12x8 well stripsWash Buffer (10X): 1x50mlDiluent (5X):1x50mlLysis Buffer (10X): 1x12mlDetection Antibody: 1x12mlDetector 100X (HRP conjugated anti-IgG): 1x150ulMouse FTO Standard (lyophilized): 20ngMouse FTO QC Sample (lyophilized): 1 vialSubstrate Solution (TMB): 1x12mlStop Solution: 1x12mlPlate Sealers: 3 eachStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到FTO, Mouse, BioAssay ELISA Kit (Alpha-ketoglutarate-dependent Dioxygenase FTO, Fat Mass and Obesity-associated Protein, Protein Fatso, Kiaa1752)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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