Carboxypeptidase B(EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kda, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate(DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.
序列
该【rCarboxypeptidase-B】重组大鼠血浆肽酶-BRecombinant Rat Carboxypeptidase-B是Prospec品牌中国区域代理商艾美捷科技产品,具体的蛋白序列可查看提供的英文版本产品说明书。
来源宿主
大肠杆菌中重组表达
溶解建议
提供的大鼠羧肽酶-B(rCarboxypeptidase-B)产品为过滤灭菌的冻干粉末;该冻干粉末是从含大鼠羧肽酶-B(rCarboxypeptidase-B)的100mM NaCl,20mM Tris pH 7.5溶液中冻干产生的.我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml,后继实验过程中可根据实验要求进一步稀释.