- 中文名称
Estriol BioAssay ELISA Kit
- 英文名字
- Estriol BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- E3560-10
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Estriol is a female hormone that exists in blood in the free and conjugated forms (primarily estriol-3-sulfate-16-glucuronide, estriol-16-glucuronide and estriol-3-sulfate). Measurement of the total estriol has been shown to be useful in monitoring the fetal function. During pregnancy, virtually all of the estriol in the maternal circulation is synthesized in the fetoplacental unit. Estriol begins to appear in the maternal circulatory system as a result of synthesis by the feto-placental unit in the ninth week of gestation. During the third trimester, estriol levels in serum and urine normally increase rapidly. Therefore, a low or declining estriol level may be indicative of fetal distress. Although a single estriol measurement may have some value as a serum test, serial determinations to monitor estriol are considered more useful.Principle of the Assay:This is an ELISA for the quantitative analysis of Estriol levels in biological fluid. This test kit operates on the basis of competition between the enzyme conjugate and the Estriol in the sample for a limited number of binding sites on the antibody coated plate.The sample or standard solution is first added to the microplate. Next, the diluted enzyme conjugate is added and the mixture is shaken and incubated at room temperature for one hour. During the incubation, competition for binding sites is taking place. The plate is then washed removing all the unbound material. The bound enzyme conjugate is detected by the addition of substrate, which generates an optimal color after 30 minutes.Quantitative test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650nm. The extent of color development is inversely proportional to the amount of Estriol in the sample or standard. For example, the absence of Estriol in the sample will result in a bright blue color, whereas the presence of Estriol will result in decreased or no color development.Kit Components: E3560-10A: ELISA Buffer 1x30mlE3560-10B: Wash Buffer 1x20ml. To be diluted 10x with deionized water. This is used to wash all unbound enzyme conjugate, samples and standards from the plate after the one hour incubation.E3560-10C: TMB Substrate 1x20ml. Stabilized 3,3', 5,5' Tetramethylbenzidine (TMB) plus hydrogen peroxide (H2O2) in a single bottle. It is used to develop the color in the wells after they have been washed.E3560-10D: Extraction Buffer, 5x 1x30ml. To be diluted 5x with deionized water. This is used for diluting extracted and non-extracted samples.E3560-10E: Estriol (HRP) 1x150ul. Estriol HRP concentrate.E3560-10F: Estriol Standard 1x100ul. Estriol standard at the concentration of 1ug/ml.E3560-10G: Microtiter Coated Plate 1x96 well Costar®microplate with anti-Estriol rabbit antibody precoated on each well. The plate is ready for use as is. Do not wash.Storage and Stability:Store all components at 4°C Stable for at least 6 months.
- 产品特点
- 针对Estriol BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Estriol BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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