- 中文名称
Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)
- 英文名字
- Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)
- 供应商
- USBiological
- 产品货号
- D8036-01
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The specificity of DHT ELISA kit was determined by measuring interference from high concentrations of the following; DHT (100%), Testosterone (8.7%), 5-beta-DHT (2%), Androstenedione (0.2%), Dehydroepiandrosterone sulfate, 17-beta-estradiol, Estriol, Estrone, Progesterone, 17-OH-Progesterone, Cortical pregnenolone were all <0.01%. DHT kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in all species, this kit should work in most species as long as the sample concentration is within the range of this kit. DHT ELISA kit is based on simultaneous binding of DHT from samples and DHT-HRP conjugate to anti-DHT immobilized on the microtiter well plates. Therefore, free DHT and enzyme-bound DHT compete for limited antibody bound to the pale. In the absence of free DHT, there is a maximum amount of DHT-enzyme bound to the plate. At the end it will produce the maximum color. As the amount of free DHT (samples or std) increases, it reduces the amount of enzyme bound to the plate. The color (blue) produced is inversely proportional to the concentration of DHT in the sample. A std. curve is constructed by generating color from no DHT (std. A, 0pg/ml DHT) to the highest std (Std E, 2500pg/ml DHT). Adding stopping solution terminates the reaction. Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of DHT in samples and control is read off the standard curve.Dihydrotestosterone (DHT) (Full name, 5a-Dihydrotestosterone, abbreviating to 5a-DHT, androstanolone) is a biologically active metabolite of the hormone testosterone, formed primarily in the prostate gland, testes, hair follicles, and adrenal glands by the enzyme 5a-reductase by means of reducing the 4,5 double-bond. Dihydrotestosterone belongs to the class of compounds called androgens, also commonly called androgenic hormones or testoids. Androgens are part of the biology of gender by stimulating and controlling the development and maintenance of masculine characteristics. DHT is 3 times more potent than testosterone; testosterone is 5-10 times more potent than adrenal androgens. While DHT is best known for its roles in causing male pattern hair loss and prostate problems, it is crucial to virilization and is necessary to mitigate estrogen's effects in men. DHT is an important contributor to other characteristics generally attributed to males, including facial and body hair growth, and deepening of the voice. Unlike other androgens such as testosterone, DHT cannot be converted by the enzyme aromatase to estradiol. It, therefore, is frequently used in research settings to distinguish between effects of testosterone caused by binding to the androgen receptor, and those caused by testosterone's conversion to estradiol and subsequent binding to estrogen receptors.Samples:Serum. DHT ELISA kit has been used in mouse/rat serum and plasma and culture medium (see published references). Other fluids may be adaptedStandards: 25-2500pg/ml Sample: 50ulSensitivity: 6pg/mlKit Components:D8036-01A: Microtiter Plate 1x96 wellsD8036-01B: Standard 0pg/ml, 1x10mlD8036-01C: Standard 25pg/ml 1x600ulD8036-01D: Standard 100pg/ml 1x600ulD8036-01E: Standard 500pg/ml 1x600ulD8036-01F: Standard 1000pg/ml 1x600ulD8036-01G: Standard 2500pg/ml 1x600ulD8036-01H: DHT Low Control, 30.4-71pg/ml 1x 600ulD8036-01J: Assay Buffer 1x15mlD8036-01K: DHT (HRP), 100X 1x200ulD8036-01L: TMB Substrate 1x16mlD8036-01M: Wash Buffer, 10X 1x50mlD8036-01N: Stop Solution (1N H2SO4)1x6mlD8036-01P: DHT High Control, 317.2-528.6pg/ml 1x600ulStorage and Stability:Store at 4°C. Stable for 6 months after receipt For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Dihydrotestosterone ELISA Kit, BioAssay (5a-DHT, Androstanolone)产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
