- 中文名称
DNA, ss Apoptosis BioAssay ELISA Kit
- 英文名字
- DNA, ss Apoptosis BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- D3878-25
- 产品报价
- ￥1/1Kit
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- The ssDNA Apoptosis ELISA Kit is a convenient, sensitive method for early detection of apoptosis. Principle:This procedure is based on the selective denaturation of DNA in apoptotic cells by formamide, and detection of denatured DNA with monoclonal antibody to single-stranded DNA (ssDNA). Formamide is a gentle agent that denatures DNA in apoptotic cells, but not in necrotic cells or in the cells with DNA breaks in the absence of apoptosis1. The sensitivity of DNA in apoptotic cells to formamide is not related to DNA breaks, but rather reflects changes in chromatin associated with apoptosis, such as chromatin condensation and digestion of proteins stabilizing DNA. The assay includes attachment of cells to 96-well plates, treatment of attached cells with formamide, and staining of ssDNA in apoptotic cells with a mixture of primary antibody and peroxidaselabeled secondary antibody. The protocol based on the one-step detection of ssDNA with antibody mixture has higher sensitivity and lower number of steps than standard two-step immunostaining2. This mixture is included in the kit in a ready to use form. The assay can be performed in two formats: 1. Cells are grown, treated with apoptosis inducing agents, stained and analyzed in the same microtiter plate. This assay is suitable for high throughput screening when induction of apoptosis is used as the endpoint of drug activity. 2. Cell suspensions obtained from cultures or tissues are transferred into a microtiter plate for staining and analysis. For this assay, cells could be fixed with methanol and stored before transfer into plates. Alternatively, fixation could be performed in microtiter plates after transfer of non-fixed cells. Total assay time is 3–4 hours, which includes fixation and staining. Only one washing step is required. The ssDNA Apoptosis ELISA is for research use only. Not for use in diagnostic or therapeutic procedures.Assay Characteristics Sensitivity:The high sensitivity of the ssDNA Apoptosis ELISA is characterized by1. Detection of 500 apoptotic cells per well and a linear relation between the absorbance and the number of apoptotic cells in the range of 500–5000 cells. 2. The ability to produce intense apoptosis signal by drug concentration inducing 50% growth inhibition, indicating that the assay sensitivity is sufficient for drug screening2. Specificity for Apoptosis over Necrosis The assay is highly specific for apoptotic cells as demonstrated by the absence of mAb reactivity with necrotic cells or with cells containing DNA breaks in the absence of apoptosis. Specificity of the assay was demonstrated using necrosis induced by hyperthermia or detergent and DNA breaks induced by hydrogen peroxide. These cells were completely negative by formamide-mAb assay, but positive by TUNEL1,2. Specificity is a major advantage of this anti-ssDNA mAb assay in comparison with the widely used TUNEL procedure. Universal Detection The detection of apoptotic cells with formamide-mAb procedure is based on the staining of condensed chromatin in apoptotic nuclei. Since chromatin condensation in compact masses is the most specific and definite hallmark of apoptosis, this method provides universal detection of apoptosis. The apoptotic events that occur without DNA breaks or without activation of specific caspases will be detected with our mAb to ssDNA. Kit Components:D3878-25A.. Antibody Mixture, 1x10ml. Ready to use. Primary monoclonal to ssDNA and HRP-labeled Anti-mouse IgMD3878-25B. Formamide, 1x5ml. Ready to use.D3878-25C. Positive Control, 1x2ml vial, 0.3ug/ml. Single-stranded DNA.D3878-25D. Wash Buffer Concentrate (10X), 1x10mlD3878-25E. ABTS,1x12ml. Ready to use solution of 2,2'-AZINO-bis (3-ethylbenziazoline-6-sulfonic acid) in a proprietary buffer with enhancer. D3878-25F. Stop Solution, 1x12ml HCl solution. Once the kit is opened, Antibody Mixture and ssDNA Positive Control should be thawed on ice, aliquoted and stored at-20º C. Formamide, Wash Buffer, ABTS Solution and Stop Solution can be thawed and stored at 4°C.
- 产品特点
- 针对DNA, ss Apoptosis BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到DNA, ss Apoptosis BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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