VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C 152S is analog to the human VEGF-C 156S mutant and only active toward VEGFR-3/FLT-4 but，unlike wild type VEGF-C，is unable to bind to and to activate signaling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells，indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding. VEGF-C，also known as Vascular Endothelial Growth Factor Related Protein （VRP），is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. The rat VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It is almost identical to the mouse VEGF-C protein. Similar to VEGF-D，VEGF-C has a VEGF homology domain spanning the middle third of the precursor molecule and long N- and C-terminal extensions. In adults，VEGF-C is highly expressed in heart，placenta，ovary and small intestine. Recombinant rat VEGF-C，lacking the N- and C-terminal extensions and containing only the middle VEGF homology domain，forms primarily non-covalently linked dimers. This protein is a ligand for both VEGFR-2/KDR and VEGFR-3/FLT -4. Since VEGFR-3 is strongly expressed in lymphatic endothelial cells，it has been postulated that VEGF-C is involved in the regulation of the growth and/or differentiation of lymphatic endothelium. Although recombinant rat VEGF-C is also a mitogen for vascular endothelial cells，it is much less potent than VEGF-A.
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来源宿主
杆状病毒系统重组表达-昆虫Sf9细胞中重组表达
溶解建议
提供的大鼠血管内皮生长因子-C（152aa）（rVEGF C （152a.a.））产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml大鼠血管内皮生长因子-C（152aa）（rVEGF C （152a.a.））的BSA溶液中冻干产生的.我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml，后继实验过程中可根据实验要求进一步稀释.
保存建议
大鼠血管内皮生长因子-C（152aa）（rVEGF C （152a.a.））在室温可保存3周，或置于-18℃冻存用于长期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装后置于-18℃冻存.请避免反复冻融.
