- 中文名称
蛋白定量用BSA标准品
- 英文名字
- BSA Standard for Protein Quantitation
- 供应商
- CellMosaic
- 产品货号
- CMC-CM92006
- 产品报价
- ¥询价/40
- 产品说明书
- 点击查看
- 购买方式
- 90%产品中国有现货库存。银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系。本公司所有产品仅供科研使用,不用于临床诊断。
- 产品新闻
- 背景资料
- Protein concentration determination is very complex and affected by many factors, such as the protein composition, conformation, and sample matrix [1]. The most often used simple method is to determine the concentration by UV absorbance at 280 nm using the Beer-Lambert Law. The 280 nm UV absorbance is mainly attributed to the number of aromatic amino acids (tryptophan and tyrosine) in the protein and is affected by protein conformation, aggregation, solvent, and pH. For many proteins, there is no experimentally determined extinction coefficient at 280 nm for the concentration determination. Other indirect protein quantitation methods, such as Bradford [2, 3] and bicinchoninic acid (BCA) assay [4] (introduced by Smith, et al. in 1985), are based on protein-dye interactions to generate a colorimetric signal. The signal also varies with protein composition and assay conditions. BSA is usually used as the standard for these assays. nnCellMosaic’s chromatographic purified BSA standard is designed for quantitation of proteins and protein conjugates by HPLC, MS, and other protein assays. This product consists of 1 mg lyophilized ultra-pure BSA that can be quickly reconstituted in 980 µL of deionized water to obtain a 1 mg/mL solution in 1x PBS buffer without any insoluble particles. CellMosaic routinely uses this product for its own internal bioconjugation-related research.nnThis product can be used together with CellMosaic’s SEC (gel filtration) HPLC Protein Standard (CM92004) to better characterize proteins and bioconjugates.
- 产品描述
- “蛋白质浓度的测定非常复杂,受多种因素影响,如蛋白质组成、构象和样品基质[1]。 最常用的简单方法是使用Beer-Lambert定律,通过280 nm处的UV吸光度测定浓度。 280 nm UV吸光度主要归因于蛋白质中芳香族氨基酸(色氨酸和酪氨酸)的数量,并受蛋白质构象、聚集体、溶剂和pH值的影响。对于许多蛋白质,在280 nm处没有用于浓度测定的实验测定消光系数。 其他间接蛋白定量方法,如Bradford [2,3]和二喹啉甲酸(BCA)测定法[4](由Smith等人于1985年引入),基于蛋白-染料相互作用产生比色信号。 信号还随蛋白质组成和测定条件而变化。 BSA通常用作这些测定的标准品。 CellMosaic的色谱纯化BSA标准品设计用于通过HPLC、MS和其他蛋白质测定法定量蛋白质和蛋白质结合物。 本品由1 mg冻干超纯BSA组成,可在980 µL去离子水中快速复溶,在1x PBS缓冲液中获得1 mg/mL溶液,无任何不溶性颗粒。 CellMosaic经常使用该产品进行内部生物结合相关研究。
- 产品形式
- 粉末或者液体
- 保存建议
- Below -20
- 其他
- CellMosaic是生物偶联技术的创新者。CellMosaic成立于2008年一直致力于开发创新的连接剂、交联技术和先进的共轭工艺。CellMosaic基于先进的偶联技术开发的商业化个性化偶联试剂盒(PerKit)让研究人员能在实验室实现高质量的生物共轭物制备,可用于ADC药物制备。生物偶联物被用作研究工具、诊断试剂,以及药物的新型载体,如抗体药物偶联物(ADCs)。n此外CellMosaic公司还率先提出了利用糖醇(SA)单体来化学组装新型交联试剂和聚合物载体的概念,称为AqueaTethe,用于共轭和药物输送。SA在结构上类似于聚乙二醇(PEG),但在亲水性、负载、多功能性和易于定制等方面具有许多优势。CellMosaic公司还开发了用于研究蛋白质相互作用的oxLin和sxLin技术,以及用于通过质谱检测和定量感兴趣分析物的微量Nelon技术。
- 注意
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该页面的中文产品信息的翻译,仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前,请浏览说明书确认。
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