2. Aminooxy group for chemical crosslinking of any biomolecule containing a ketone or aldehyde group. Aminooxy-ketone ligation is orthogonal to peptide chemistry and will allow highly selective solid phase-based separation of the aminooxy-labeled crosslinked products. In particular, the selective purification method will permit the characterization of protein complexes in complex matrices, such as plasma, cellular membranes, and cell lysates, where these samples may contain free thiols that interfere with the labeling and purification processes.
3. Hydrophilic AqT® linkers: TFMP group is highly hydrophobic, biomolecules labeled with a TFMP using a traditional ethylene and ethylene glycol-type linker tend to aggregate and destabilize the labeled protein. T2A10 AqT® linker is made by linking two threitols together, greatly increasing the hydrophilicity of oxLink™ and its water solubility (>27 mg/mL). The molecule is also more biocompatible with decreased non-specific hydrophobic interactions with other biomolecules, allowing high loading of phenyldiazirine groups.
The product is sold as a lyophilized powder in 1 vial of 250 µg (Cat# CM81402-250UG) or 4 vials of 250 µg (Cat# CM81402-4x250UG).
