This kit is designed to label any IgG antibody with a VC-PAB linker. The final labeled product can be used as a negative control for any ADC with VC-PAB linker via thiol labeling chemistry. The preparation is essentially the same as standard ADC preparation with VC-PAB linker. The user supplies the antibody and the kit includes maleimide-activated VC-PAB-OH, which can be coupled directly to the antibody after reduction through alkylation in a single step (a method developed by Seattle Genetics: Sun et al. 2005, Bioconjugate Chem. 16, 1282-1290). The kit provides materials to then purify the ADC control by removing any unreacted linkers.
This kit provides materials to conjugate 1-3 mg of one (CM11429x1) or three (CM11429x3) antibody samples (IgG).