- 中文名称
C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)
- 英文名字
- C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)
- 供应商
- USBiological
- 产品货号
- C7905-06
- 产品报价
- ￥1/96Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- C-Peptide is part of the molecule of Proinsulin, that consists of three parts: C-Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C-Peptide are produced. C-Peptide is released into the blood stream in equal amounts to insulin. A test of C-Peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Although C-Peptide function is not fully understood, it binds to cell membranes which leads to increased intracellular Ca2++, K+ -ATPase and endothelial nitric oxide synthase activities. C-Peptide also functions in repair of the muscular layer of arteries.C-peptide assay measures C-peptide in human serum or other biological fluids.Description:Human insulin and C-Peptide originate as a single polypeptide chain known as proinsulin(MW 9000) in the pancreatic cell. Proinsulin is cleaved proteolytically to form equimolar amounts of mature insulin and C-Peptide that are released into the portal vein. So called because it connects the A and B chains of insulin in the proinsulin molecule. C-Peptide is a single chain of 31 amino acid (MW 30,200). Unlike insulin has no known physiological function. Because C-Peptide has a longer half-life than insulin (2-5 times longer), high concentrations of C-Peptide persist in the peripheral circulation and these level fluctuate less insulin. For these reasons, in plasma C-Peptide concentrations may reflect pancreatic insulin secretion more reliable than the level of insulin itself1. C-Peptide is cleaved from the body by the kidney. Urine concentrations of C-Peptide are 20-50 times higher than in plasma, unlike plasma insulin levels, which fluctuate in response to meals, measurement of the 24 hour urinary excretion of C-Peptide provides a useful monitor of average cell insulin secretion 2. C-Peptide measurements are useful in insulinoma diagnosis, especially in patients treated with insulin. Elevated C-Peptide levels are indicative of insulinoma. C-Peptide measurements are useful in the need for progression to insulin therapy in non-insulin dependent diabetics (NIDDM). C-Peptide measurements are useful as a marker for residual pancreatic tissue after pancreatectomy. It may also be used to monitor the progress of pancreas or islet cell transplantation. C-Peptide measurements are useful in the diagnosis of hypoglycemia brought on by surreptitious insulin administration.Expected Values:It is recommended that each laboratory must determine its own normal and abnormal ranges. Reference Range:A healthy normal population of 100 samples were assayed (0.2-13.2ng/ml).Fasting: 1-3ng/mlSpecificity:High concentrations of lipid or bilirubin do NOT interfere in the insulin assay. Purified hemoglobin up to 50ug/ml has been shown not to interfere the test. No interference for rheumatoid factor or human anti-mouse antibodies (HAMA) was observed. Additional crossreactivity was determined as follows:Human pro-insulin (32- 33 split): 63%Human pro-insulin (64-65 split): 87%Human insulin: 0%Human C-Peptide of Insulin: 100%Fasting concentrations of intact and split pro-insulin are typically only 1-2% of C-Peptide concentrations. Crossreactivity with these molecules is not clinically significant.Species Crossreactivity:This kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Applicability for animal samples will depend upon the crossreactivity of the human antibodies used in the kit with a given animal's hormones/proteins. C-peptide assay measures C-peptide in human serum or other biological fluids.Kit Components:C7905-06A: Microtiter Plate 1x96wellsC7905-06B: Assay Buffer 1x16ml*C7905-06C: Standard 0ng/ml 1x2ml*C7905-06D: Standard 0.3ng/ml1x600ul*C7905-06E: Standard 0.5ng/ml1x600ul*C7905-06F: Standard 2ng/ml1x600ul*C7905-06G: Standard 8ng/ml1x600ul*C7905-06H: Standard 16ng/ml1x600ul*C7905-06J: Control, Low 0.885-1.476ng/ml 1x600ul*C7905-06K: Control, High 5.85-9.75ng/ml 1x600ulC7905-06L: Stop Solution, 1N H2SO4 1x6mlC7905-06M: Wash Solution, 10X 1x50mlC7905-06N: TMB Substrate Complete 1x16mlC7905-06P: C-Peptide (HRP), 100X 1x300ulNOTE: Standards and controls are not stable at 4°C. Aliquot and store at -20°C.Storage and Stability:Store all components except standards and controls at 4°C. Aliquot and store standards C7905-06C - C7905-06H and controls C7905-06J and C7905-06K at -20°C. Stable for a 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 产品特点
- 针对C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到C-Peptide ELISA Kit, Human, BioAssay (Hyperproinsulinemia, Insulin Precursor, Proinsulin)产品后将其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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