- 中文名称
Copper Assay Kit, BioAssay
- 英文名字
- Copper Assay Kit, BioAssay
- 供应商
- USBiological
- 产品货号
- C7900-59K
- 产品报价
- ￥1/250Tests
- 产品说明书
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- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper is mainly present in caeruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anaemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson's disease, microcytic hypochromic anaemia and bone disease due to reduced collagen synthesis.Simple, direct and automation-ready procedures for measuring copper concentrations find wide applications in research, drug discovery and environmental monitoring. Copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured at 359nm, is directly proportional to copper concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.Key Features:Sensitive and accurate. Linear detection range 7 ug/dL (1.0uM) to 300ug/dL (47uM) copper in 96-well plate assay.Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.Applications:Direct Assays: biological, environmental, food and beverage samples.Drug Discovery/Pharmacology: effects of drugs on Cu metabolism.Kit Contents: (250 tests in 96-well plates)Reagent A: 10ml Reagent B: 1.5ml Reagent C: 40mlCopper Standard: 1ml 1.5 mg/dL Cu2+Storage conditions. The kit is shipped at room temperature. Store all reagents at 4 °C. Shelf life: 12 months after receipt. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.Procedures:Note: metal chelators (e.g. EDTA) interfere with this assay and should be avoided in sample preparation.Procedure using 96-well plate:1. Standards: transfer 100ul dH2O into one Eppendorf tube labeled “Blank”. Into another tube labeled “Standard”, mix 20ul 1.5 mg/dL Standard and 80ul dH2O (final 300 ug/dL Cu2+). Samples: transfer 100ul samples into separate tubes. Add 35ul Reagent A (trichloroacetic acid) to each tube and mix by vortexing. If samples contain protein (e.g. serum/plasma), precipitates form. Centrifuge tubes for 2 min at 14,000 rpm and use clear supernatant for assay. For samples that do not contain protein, the mixture remains clear and centrifugation is not necessary. Transfer 100ul Blank, Standard and Sample into separate wells of a clear flat-bottom 96-well plate.2. For each assay well, prepare Working Reagent by mixing 5ul Reagent B and 150ul Reagent C. Transfer 150ul Working Reagent to each well and tap plate to mix thoroughly.3. Incubate 5 min at room temperature and read optical density at 356-362nm (peak absorbance at 359nm).Note: if sample OD values are higher than the OD value for the 300ug/dL Standard, dilute sample in dH2O and repeat assay. Multiply the results by the dilution factor.Procedure using cuvette:Prepare standards and samples as for 96-Well Assay Procedure::.1. Transfer 400ul Standards and Samples into separate cuvets.2. Add 600ul Working Reagent. Mix by pipetting.3. Incubate 5 min at room temperature and read optical density at 356-362nm (peak absorbance at 359nm).Calculation:The copper concentration of Sample is calculated as=ODSAMPLE–ODBLANKODSTANDARD–ODBLANK× 300 (ug/dL)ODSAMPLE, ODBLANK and ODSTANDARD are optical density values of the Sample, Blank and the 300 ug/dL Standard, respectively.Conversions: 100 ug/dL Cu equals 15.5uM, 0.0001% or 1 ppm.Materials Required, But Not Provided:Pipeting devices and accessories. For 96-well plate assays: clear flat-bottom 96-well plates and plate reader. For cuvet assays: spectrophotometer and cuvets for measuring OD at 356-362nm.General Considerations:For scarce samples (e.g. mice serum or plasma), mix sample with dH2O to a total of 100ul, e.g. 50ul serum+50ul dH2O. Multiply the results by the dilution factor (2 fold).Examples:Human serum, rat plasma, rat serum, and bovine serum were assayed in duplicate using the 96-well plate assay protocol. The copper concentrations were 97 ± 1, 104 ± 1, 101 ± 2, 78 ± 1 ug/dL, respectively.
- 产品特点
- 针对Copper Assay Kit, BioAssay该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Copper Assay Kit, BioAssay产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
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- 注意
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该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
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