- 中文名称
Chorionic Gonadotropin, Intact, Human (hCG) BioAssay ELISA Kit
- 英文名字
- Chorionic Gonadotropin, Intact, Human (hCG) BioAssay ELISA Kit
- 供应商
- USBiological
- 产品货号
- C5069-15
- 产品报价
- ￥1/96Tests
- 产品说明书
- 点击查看
- 购买方式
- 银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868
- 产品新闻
- 背景资料
- USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！
- 产品描述
- Human chorionic gonadotropin is a glycoprotein hormone produced by normal trophoblast cells of the placenta during pregnancy. It is also is produced by trophoblast cells in hydatidiform mole and choriocarcinoma (trophoblast diseases), and in patients with germ cell tumors (testicular choriocarcinoma, placental site tumors and germ cell carcinomas of the ovary) and sometimes in those with other malignancies. Small amount of hCG may also be produced by the pituitary gland. Human CG (hCG) is the hormone associated with the maintenance of pregnancy.Human chorionic gonadotropin is a member of the glycoprotein hormone (GPH) family. Like the other glycoprotein hormones, follicle stimulating hormone (FSH), luteinizing hormone (LH) and thyroid stimulating hormone (TSH), hCG is composed of two subunits, alpha and beta. Alpha subunits of these various glycoprotein hormones are structurally very similar, but beta subunits differ in amino acid sequences. These differences are responsible for their biological and immunological specificity. The alpha-subunit of hCG is a glycopeptide of 92 amino acids and the beta-subunit of hCG is a glycopeptide of 145 amino acids. HCG has a molecular weight of 50,000 daltons, consists of an alpha subunit of 18,000 daltons and a beta subunit of 32,000 daltons. Recent elucidation of the crystal structure of hCG has revealed that all these subunits share the so-called cystine-knot structural motif with growth factors such as nerve (NGF). platelet-derived (PDGF). Although the pituitary secretes three related glycoprotein hormones, LH, FSH, and TSH, hCG is the only one to produced by the placenta in primates to maintain the steroid hormone secretions of the corpus luteum. Predominantly intact hCG is present in serum and urine samples in normal pregnancy, as well as in hydatidiform mole. In women with extrauterine or ectopic pregnancies, unduly low hCG levels may be detected. Variable levels of hCG mostly intact hCG, may be present from individuals with persistent trophoblastic disease. Elevated concentrations are also present in cases of early pregnancy loss (EPL) or biochemical pregnancy, gestational Down syndrome. Bladder cancer, ovarian cancer and certain other malignancies may generate a small amount of hCG alpha and beta subunit. Commonly, the amount of subunit is insufficient for combination to occur to make intact hCG.Principle:This hCG enzyme linked immunosorbent assay (ELISA) applies a technique called aquantitative sandwich immunoassay. The microtiter plate provided in this kit has been precoated with a monoclonal antibody specific to hCG. Standards or samples are added to the microtiter plate wells and incubated. The plate wells are washed to remove any unbound standard or sample, hCG, if present, will bind to the antibody pre-coated on the wells. A standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific to hCG is added to each well to “sandwich” the hCG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3', 5,5' Tetramethyl-benzidene) substrate solution is added to each well. This enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hCG and enzyme-conjugated antibody will exhibit a change in color. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and thecolor change is measured spectrophotometrically at a wavelength of 450nm. In order to measure the concentration of hCG in the sample, this ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus hCG concentration (mIU/ml). The concentration of hCG in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <3.0mIU/mlRange: 7.5-240mIU/mlKit Components:C5069-15A HCG Microtiter Plate 1x96 wells. Pre-coated with anti-human HCG monoclonal antibody.C5069-15B HCG Pab (HRP) 1x12mlC5069-15C Standard-240 mIU/ml 1x1vialC5069-15D Standard-120 mIU/ml 1x1vialC5069-15E Standard-60 mIU/ml 1x1vialC5069-15F Standard-30 mIU/ml 1x1vialC5069-15G Standard-7.5 mIU/ml 1x1vialC5069-15H Standard-0 mIU/ml1x1 vialC5069-15J Substrate A 1x10mlC5069-15K. Substrate B 1x10mlC5069-15L. Stop Solution 1x14ml, 2N H2SO4. C5069-15M. Sample Diluent 1x10mlStorage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 产品特点
- 针对Chorionic Gonadotropin, Intact, Human (hCG) BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。
- 保存建议
- 建议收到Chorionic Gonadotropin, Intact, Human (hCG) BioAssay ELISA Kit产品后将其置于4°C保存。
- 其他
- Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。
-
- 注意
-
该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。
-
