TCEP (Tris(2-carboxyethyl)phosphine) hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter. Unlike DTT, TCEP hydrochloride retains its reducing power at acid pH (pH 5) and at pH above 7.5. TCEP hydrochloride is unreactive towards other functional groups found in proteins. For reducing disulfide bonds in peptides or proteins, 5-50 mM TCEP can be used for a few minutes at room temperature. Molecular Information
C9H15O6P HCl
Mwt: 286.64
[51805-45-9]
产品描述
TCEP(三(2-羧乙基)膦)盐酸盐是一种无味的还原剂,比二硫苏糖醇(DTT)或2-巯基乙醇更稳定和有效。TCEP盐酸盐可溶于水至310克/升。与DTT不同,TCEP盐酸盐在酸性pH(pH 5)和pH高于7.5时保留其还原能力。TCEP盐酸盐对蛋白质中发现的其他官能团不反应。为了还原肽或蛋白质中的二硫键,可以在室温下使用5-50 mM TCEP几分钟。nbsp; 分子信息