MitoView™ 633，试模尺寸-MitoView™ 633, Trial Size-荧光染料-艾美捷科技有限公司

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背景资料: MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence. MitoView™ 633 is a far-red fluorescent mitochondrial dye with absorbance/emission at 622/648 nm. The dye is membrane permeable and becomes brightly fluorescent upon accumulation in the mitochondrial membrane. Staining is dependent on mitochondrial membrane potential, and can be used to monitor mitochondrial membrane potential in intact cells. The dye is designed for use in live cells, and is not fixable. Note: The optimal detection settings for MitoView™ 633 are the same as for Cy®5 and other far-red dyes. However, the dye also has visible red fluorescence and can be imaged using Cy®3 settings as well. As a consequence, the dye cannot be used for two-color imaging with other red probes. In addition to MitoView™ dyes, Biotium also offers several classical dyes for measuring mitochondrial membrane potential. These include JC-1, a ratiometric dye that forms red aggregates in healthy cells, which are reduced to the green monomeric state when mitochondrial membrane potential is lost. We also offer TMRM and TMRE, two red fluorescent dyes that are used to quantitatively measure mitochondrial membrane potential using the Nernst equation. Cy Dye is a registered trademark of GE Healthcare.

产品描述: MitoView™染料是用于活细胞的荧光线粒体染色剂。染料在线粒体中快速积累，并且可以在不洗涤的情况下成像。它们有蓝色、绿色、远红色和近红外荧光。nbsp; MitoView™633是一种远红荧光线粒体染料，在622/648 nm处具有吸收/发射。染料是膜可渗透的，并且在线粒体膜中积累时变得明亮荧光。染色依赖于线粒体膜电位，并且可用于监测完整细胞中的线粒体膜电位。该染料设计用于活细胞，并且不可固定。注：MitoView 633的最佳检测设置™与Cy®5和其他远红染料相同。然而，染料也具有可见的红色荧光，并且也可以使用Cy®3设置成像。因此，该染料不能与其他红色探针一起用于双色成像。nbsp; 除MitoView™染料外，Biotium还提供几种用于测量线粒体膜电位的经典染料。这些包括JC-1，一种在健康细胞中形成红色聚集体的比率染料，当线粒体膜电位丧失时，红色聚集体被还原为绿色单体状态。我们还提供TMRM和TMRE，这两种红色荧光染料用于使用能斯特方程定量测量线粒体膜电位。nbsp; Cy Dye是GE Healthcare的注册商标。

其他: Biotium公司是一家在荧光技术领域独树一帜的生物技术公司，公司位于美国加利福尼亚的BAY AREA------中外知名首要的生物科技中心。该公司一直致力于开发和生产用于生物医学研究的荧光指示剂及其他特殊生化产品。其产品被广泛应用于细胞生物学、蛋白质组学、分子生物学、免疫学、微生物学、诊断学、生物化学、神经科学、血液流动检测及大规模药物筛选等众多领域。Biotium公司专利产品众多，包括已经被广泛使用的实时PCR定量荧光染料EvaGreen、具有划时代意义的凝胶核酸染料Gel Red和GelGreen，以及最新推出的Cheetah Taq热启动酶和CF系列蛋白质标记染料等。 n目前，Biotium已将其许多专利技术授权给一些主要的国际生物技术公司，包括安捷伦、BD生物科学、Biolegend、BioRad、EMD Millipore、Illumina、PerkinElmer、Qiagen、Roche、Sartorius AG、Sigma-Aldrich、Thermo Fisher Scientific等。