Biotium developed Neuro-DiO as a replacement for the green fluorescent DiO, which has been found to be difficult to use for neurons and cell suspension due to low solubility, tendency to form aggregates and slow lateral diffusion rate. Neuro-DiO and DiO have nearly identical absorption and emission spectra, but the former has better solubility in membranes and does not form nonfluorescent aggregates, which also tend to slow down the dye diffusion rate in membranes. Please also see our ready-to-use CellBrite™ Cell Membrane Labeling Kits.
&lamba;Ex/&lamba;Em = 484/501nm
Yellow-orange solid soluble in ethanol, hexane, DMSO or vegetable oil
