Sulfo-Cy3-NHS is a fluorophore featuring a sulfonate group and an activated NHS ester. NHS esters are highly reactive towards nucleophiles such as amines to form stable amide bonds. Cy3 is a cyanine dye with excitation and emission maxima at 555 nm and 570 nm respectively. The sulfonate group on the Cy3 dye increases this compound’s water solubility for easy use in vivo.