With the new Arf6 G-LISA® kit you can now measure Arf6 activation from cell and tissue samples in less than 3 h. G-LISA® requires only 1-5% of the material needed for a conventional pull-down assay. You will also be able to handle large sample numbers and generate quantitative results. For a more detailed introduction on G-LISA® assays and a listing of other available G-LISA® kits, see our main G-LISA page.
The Arf6 G-LISA® kit contains an Arf-GTP-binding protein linked to the wells of a 96 well plate. Active, GTP-bound Arf6 in cell/tissue lysates will bind to the wells while inactive GDP-bound Arf6 is removed during washing steps. The bound active Arf6 is detected with a Arf6 specific antibody. The degree of Arf6 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.
