Recombinant PreScission Protease (rPSP) from human rhinovirus (HRV 3C) is a recombinant restriction-grade protease. PSP is a highly purified recombinant 6xHis-fusion protein, which recognizes the same cleavage site as the native enzyme. It is used to cleave affinity tags from fusion proteins. The optimum recognition site for this enzyme is the sequence Leu-Glu-Val-Leu-Phe-Gln|Gly-Pro(LEVLFQ|GP) and cleavage occurs between the Gln and Gly-Pro residues. The optimal temperature for cleavage is 30
序列
0
来源宿主
Escherichia coli.
溶解建议
Reconstituted in ddH2O or PBS at 100 μg/ml.
保存建议
Recombinant PreScission Protease (rPSP) remains stable up to 1 year at -20
