UltraScence Femto Plus Western Substrate,作为一种基于发光氨的增强化学发光底物,是敏感的,与辣根过氧化物酶(HRP)偶联二抗体进行免疫印迹兼容。UltraScence Femto Plus Western底物的设计目的是检测数量太小的目标蛋白,用典型的ECL底物看不到。UltraScence Femto Plus Western Substrate的卓越灵敏度和长信号持续时间使抗原的低飞图检测(中飞波)成为可能。此外,它的长化学发光信号持续时间使数字和基于薄膜的成像不损失任何信号成为可能。建议适当的一抗和二抗稀释以获得最佳的信号强度和持续时间。此外,UltraScence Femto Plus WesternSubstrate的高稳定性使其在室温下可以存储2年,而不会对性能产生任何影响。
产品描述
The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate,is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugatedsecondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the targetproteins in amounts that are too small to be seen with typical ECL substrates. The low femtogram detection (midzeptomole) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and longsignal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imagingpossible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested forattaining optimal signal intensity and duration. Moreover, the high stability of UltraScence Femto Plus WesternSubstrate makes a 2-year- storage at room temperature possible, without any compromise on the performance.