OneStep Blocker is a blocking solution for Western blot analysis. This OneStep Blocker buffer not only provides blocking and primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the three-in-one step procedure, OneStep Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.