The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.
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Bovine milk may contain two types of beta-casein protein, A2 or A1, which differ by a single-point mutation at amino acid position 82 (P82/H82). Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes. The Biosensis Bovine A1 Beta Casein ELISA Kit is a sandwich ELISA that allows the specific quantification of the A1 isoform of bovine beta casein in bovine milk, only if used as directed. The A2 isoform is not detected in this ELISA assay. Please refer to the kit protocol for specific use instructions.