For quantitative detection of rat HGF in cell culture supernatants, serum, and citrate, or EDTA treated plasma samples only when used as directed The rat Hepatocyte Growth Factor (HGF) Kit is a sandwich ELISA. The capture antibody is a monoclonal mouse HGF antibody pre-coated onto the 96-well strip plates provided in the kit. Standards (expression system for standard: NSO; Immunogen sequence: Q33-R495 (alpha) and V496-L728 (beta)) and test samples are added to the wells. A biotinylated detection polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of HGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse HGF in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
产品特点
该ELIAS试剂盒包含：1 x 96孔预包被条孔板（可拆卸）, 蛋白质标准品，检测试剂，底物缓冲液，以及详细的实验方案。
