The mouse Vascular endothelial growth factor (VEGF) Kit is a sandwich ELISA. The capture antibody is a VEGF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known VEGF concentration are added to these wells and allowed to complex with the bound VEGF antibody. A biotinylated anti-mouse VEGF antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse VEGF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. Vascular endothelial growth factor (VEGF) is a disulfide linked homodimeric protein associated with angiogenesis, vasculogenesis and endothelial cell growth. At least 10 isoforms of VEGF are produced by alternative splicing with varying levels of expression. The isoforms VEGF188, VEGF164 and VEGF120 are widely expressed whereas the isoforms VEGF205 and VEGF144 are uncommon. Isoforms VEGF165 and VEGF120 are freely secreted whereas isoform VEGF188 remains cell associated.
产品特点
灵敏度:15.6 pg/mL - 1000 pg/mL。此外该ELIAS试剂盒包含：2 x 96孔预包被条孔板（可拆卸）, 蛋白质标准品，检测试剂，底物缓冲液，以及详细的实验方案。
