The human Interleukin 8 (IL-8) Kit is a sandwich ELISA. The capture antibody is a monoclonal human IL-8 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IL-8 concentration are added to these wells and allowed to complex with the bound IL-8 antibody. A biotinylated human IL-8 antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IL-8 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IL-8 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
