The rat GDNF Kit is a sandwich ELISA. The capture antibody is a monoclonal anti-rat GDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known GDNF concentration are added to these wells and allowed to complex with the bound GDNF antibody. A biotinylated ant-rat GDNF polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of GDNF present in the samples. The purpose of this kit is for quantitative detection of rat GDNF in cell culture supernatants, cell lysates, tissue homogenates, serum, and heparin or EDTA treated plasma samples only when used as directed. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. GDNF is a glycosylated, disulfide-bonded homodimer molecule. It was first discovered as a potent survival factor for midbrain dopaminergic neurons and was then shown to rescue these neurons in animal models of Parkinson's disease. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. FUNCTION: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
产品特点
灵敏度:31.2 pg/mL - 2000 pg/mL。此外该ELIAS试剂盒包含：1 x 96孔预包被条孔板（可拆卸）, 蛋白质标准品，检测试剂，底物缓冲液，以及详细的实验方案。
