sFAS is a single-pass type I membrane protein and a member of the TNF-receptor superfamily. The interaction between this receptor and the sFAS ligand (TNFSF6/FASLG) forms a death-inducing signalling complex that includes Fas-associated death domain protein (FADD), caspase 8 and caspase 10. Processing of the caspases in this complex leads to apoptosis.
产品描述
The human soluble FAS Kit is a sandwich ELISA. The capture antibody is a monoclonal human sFAS antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known sFAS concentration are added to these wells and allowed to complex with the bound sFAS antibody. A biotinylated human sFAS polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of sFAS present in the samples. The purpose of this kit is the in-vitro quantitative determination of human sFAS in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
产品特点
灵敏度:31.2 pg/mL - 2000 pg/mL。此外该ELIAS试剂盒包含：2 x 96孔预包被条孔板（可拆卸）, 蛋白质标准品，检测试剂，底物缓冲液，以及详细的实验方案。
