FUNCTION: Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) (By similarity).
产品描述
The human BDNF Kit is a sandwich ELISA. The capture antibody is a monoclonal BDNF antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known BDNF concentration are added to these wells and allowed to complex with the bound BNDF antibody. A biotinylated BDNF antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of BDNF present in the samples. The purpose of this kit is the in-vitro quantitative determination of human BDNF in samples such as sera, plasma and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
产品特点
灵敏度:31.2 pg/mL - 2000 pg/mL。此外该ELIAS试剂盒包含：2 x 96孔预包被条孔板（可拆卸）, 蛋白质标准品，检测试剂，底物缓冲液，以及详细的实验方案。
