The human Activin A Kit is a sandwich ELISA. The capture antibody is a monoclonal Activin A antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Activin A concentration are added to these wells and allowed to complex with the bound Activin A antibody. A biotinylated Activin A monoclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Activin A present in the samples.
产品特点
灵敏度:15.6 pg/mL - 1000 pg/mL。此外该ELIAS试剂盒包含：1 x 96孔预包被条孔板（可拆卸）, 蛋白质标准品，检测试剂，底物缓冲液，以及详细的实验方案。
