The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA's are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA's, primarily Leu, can stimulate insulin secretion. The BCAA's have also been implicated in a wide range of other physiological effects. This BCAA Assay Kit provides a simple convenient means of measuring the BCAA's in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reacts with probe to generate a colored product (Lmax = 450nm). The BCAA kit measures BCAA's in the range of 0.2-10nmol per sample with a detection limit of ~0.2nmol (~10uM) BCAA in sample.Kit Components:1. BCAA Assay Buffer: 1x 25ml2. BCAA Enzyme Mix: lyophilized 3. WST Substrate Mix: lyophilized 4. Leu Standard: 1x10umol (lyophilized)Storage and Stability:Store kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
