PE-Texas Red is a tandem fluorescent dye composed of PE and Texas Red. Antibodies labeled with PE-Texas Red are compatible with flow cytometers equipped with a 488nm argon laser. PE-Texas Red has a maximum emission wavelength of 615nm, which can be detected in the FL3 channel of a flow cytometer. Antibodies labeled with PE-Texas Red can be used in small flow cytometers with milliwatt-level lasers and large flow sorters equipped with full-power lasers. Activated PE is easily conjugated to antibodies and other proteins without the need for chemical crosslinkers. These highly purified PE conjugates retain their spectral properties after conjugation. Our activated PE is treated with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC), which reacts with lysine residues. The maleimide group can react with free sulfhydryl groups of the conjugate partner protein. They are ready-to-use and can be directly conjugated without further pre-processing when mixed with sulfhydryl-containing targets.
产品描述
PE-Texas Red 是由 PE 和 Texas Red 组成的串联荧光染料。PE-Texas Red 标记的抗体与配备 488nm 氩激光器的流式细胞仪兼容。PE-Texas Red 的最大发射波长为 615nm，可在流式细胞仪的 FL3 通道中检测到。用 PE-Texas Red 标记的抗体可用于配备毫瓦级激光器的小型流式细胞仪和配备全功率激光器的大型流式细胞仪。活化 PE 很容易与抗体和其他蛋白质偶联，无需化学交联剂。这些高度纯化的 PE 偶联物在偶联后仍可保留其光谱特性。我们的活化 PE 经过琥珀酰亚胺基 4-（N-马来酰亚胺甲基）环己烷-1-羧酸盐 （SMCC） 处理，它与赖氨酸残基反应。马来酰亚胺基团可与偶联伴侣蛋白的游离巯基反应。它们是即用型，与含巯基的靶标混合时，无需进一步预处理即可直接偶联。
