GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
应用类型
Dot Blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB)
免疫原
KLH-conjugated peptide from the N-terminal och the GFP proten from the jellyfish Aequorea victoria
来源宿主
小鼠
反应性
Native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
